ides around the cell surface. To assess the quantity of Alca protein around the cell surface, we performed a surface biotinylation assay. N-terminal FLAG-tagged Alca protein was expressed in CAD cells. For comparison, constitutively cleaved FLAG-tagged APP protein was expressed in parallel. To equalize the volume of expressed FLAG-tagged proteins inside the cells, the ratio with the transfected ex
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atio by lovastatin. The fact that lovastatin regulated GSK-3b and CRMP-2 is compatible to clarify its antiMFS impact. For that reason here we propose that the abnormal neuronal fiber development in TLE animal model is possibly caused by SE induced GSK-3b phosphorylation. Though the connection in between the activation of GSK-3b/CRMP-2 and MFS is just not nicely established, the truth that administ
in and presenilin 1 transgenes. Nat Med four: 97100. Borchelt DR, Lee MK, Gonzales V, Slunt HH, Ratovitski T, et al. Accumulation of proteolytic fragments of mutant presenilin 1 and accelerated amyloid deposition are co-regulated in transgenic mice. Neurobiol Aging 23: 171177. Radde R, Bolmont T, Kaeser SA, Coomaraswamy J, Lindau D, et al. Abeta42-driven cerebral amyloidosis in transgenic mice rev
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Pancreas and liver samples have been vigorously homogenized with 2.five mL of MeOH/water/HCl working with an IKA T10 Ultra-Turrax at 24000 rpm for 1 min after which exposed to ultrasound for 10 min. The mixture was centrifuged at 2880 g for five min. The methanolic phase was evaporated in a SpeedVac concentrator equipped having a refrigerated vapor trap RVT4104 and also a vacuum pump v-710, then d
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