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ve than PthA2 to inhibit the CsCyp activity. This observation is in line with our preceding research around the interaction of CsCyp with RD2 and five.5 rep+CT. Provided that PthA2 inhibited the CsCyp activity and that CsA disrupted the CsCyp-CTD interaction, we tested regardless of whether CsA could also disrupt the PthA2-CsCyp complex. Certainly, CsA prevented PthA2 from binding to CsCyp in GSTp
in feederfree cultures on Matrigel-coated six-well plates with mTeSRTM1. For typical passage, hES cells were treated with 1 mg/mL of dispase for 5 min, collected having a cell scraper and plated. For virus transduction, cells have been treated with 1 mg/mL Accutase for 5 10 min till the colonies had been dissociated into single cells. The single-cell colonies had been then detached and plated with
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