the United States and is the eighth leading cause of death from cancer among American women [1]. Endometrial cancers (ECs) account for the vast majority of uterine cancers. Endometrioid, serous, and clear cell carcinomas represent the three major histological subtypes of EC. Each subtype arises from distinct precursor lesions, has distinct clinical behaviors and distinct molecular etiologies [2],
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ter. To the cell suspension, 1 ml of TBA reagent (0.25 M HCl, 15 [w/vol] trichloroacetic acid, 0.375 [w/vol] TBA) was added and the reaction was terminated. The mixture was boiled at 100 for 15 min in a water bath and allowed to cool at room temperature. Cell debris was removed by centrifugation at 3000 ?g for 5 min and the TBARS was assayed in the supernatant at 535 nm. TBA reagent mixed with
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The primers used for miR-577 (part number 4408995) and RNU6B (RNA, U6 small nuclear 2, 4373381) were purchased from Applied Biosystems. As an endogenous control, RNU6B was amplified in parallel, and the Ct value of miR-577 was normalized to that of RNU6B. The expression levels of the tumors were compared with matched normal mucosae, which were adjusted to 1.and stained using the HistoGene LCM Froz
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ribed to generate models of acute primary and progressive secondary RVPO respectively [14,19]. Briefly, mice were intubated using a 24G angiocath and mechanically ventilated (Harvard Apparatus) at 95 breaths per minute with a tidal volume of 0.3 mL with 2.0?.5 Isoflurane and 100 flow-through oxygen. Depth of anesthesia was monitored by assessing palpebral reflex, toe pinch, respirations, and gen
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